Table 5.

CMS5 and Meth A epitopes with highest DAI scores

GeneMut/WT sequenceMut/WT ScoreDAIMeasured IC50 for Kd (Mut/WT)ICSELISpotTumor rejection
CMS5 Epitopes
Dhx8.1P(y/D)LTQYAIIML9.3/-2.311.62192/1653-+++++-
Alkbh6.1D(y/D)VPMEQP4.3/-7.311.660858/−---
Dhx8.2P(y/D)LTQYAI9.9/-1.611.5a/−-+++++-
Dhx8.3P(y/D)LTQYAII9.3/-2.211.5244/1418++++++-
Alkbh6.2D(y/D)VPMEQPR6.8/-4.711.5−/51229-+++
Dhx8.4P(y/D)LTQYAIIM6.6/-4.911.56571/6256-+++-
Alkbh6.3D(y/D)VPMEQPRP4.8/-6.711.5−/23570---
Alkbh6.4D(y/D)VPMEQPRPP4.7/-6.811.547053/2957---
Rangap1SEDKIKAI(l/P)1.4/-5.46.8−/5108-+++++-
Stau1.1LKSEEKT(l/P)0.6/-6.26.8−/−---
Stau1.2KPALKSEEKT(l/P)0.1/-6.76.8−/−---
Stau1.3PALKSEEKT(l/P)1.3/-5.46.769546/−---
Stau1.4ALKSEEKT(l/P)1.2/-5.56.7−/−--+
9430016H08RikSWSSRRSLLG(l/R)5.9/-0.66.5−/−-+++++-
Slit3GFHGCIHEV(l/R)4.7/-1.86.551640/−+++++
Atxn10.1QVFPGLME(l/R)3.4/-3.16.5−/7054+++++
Sipa1l3TTTPGGRPPY(l/R)2.7/-3.86.5−/−-++++-
Atxn10.2VFPGLME(l/R)2.5/-46.5−/1107--+
Ccdc136ELQGLLEDE(l/R)2.4/-4.16.5−/4537--+
Mast2KLQRQYRSPR(l/R)2.2/-4.36.510107/8511---
Meth A Epitopes
Tnpo3.1(sy/LD)MLQALCI8.2/-5.213.482/146+++++++
Tnpo3.2(sy/LD)MLQALCIPT7.1/-6.313.49964/85++++-
Tnpo3.3(sy/LD)MLQALCIP3/-10.413.467/111++++++-
Tnpo3.4(sy/LD)MLQALC5.9/-7.413.314927/89---
Trim26.1A(y/D)ILAALTKL12.8/1.211.6622/1.1-+++++-
Nus1P(y/D)LVLKFGPV10.5/-1.111.62359/1.9---
Tpst2.1L(y/D)EAGVTDEV10.3/-1.311.660/−-+++-
Fiz1H(y/D)LQGSNA10.3/-1.311.62473/−---
Kdm4bL(y/D)HTRPTAL10/-1.611.6264/−---
Dis3l2.1I(y/D)GVVARNRAL9.3/-2.311.6143/−++-
Ube4a.1A(y/D)AKQFAAI9.3/-2.311.612/−++-
NcdnS(y/D)CEPALNQA8.9/-2.711.6664/−+--
GapdhV(y/D)LTCRLEKPA8.9/-2.711.61150/−---
Ckap5K(y/D)MSMLEER8.1/-3.511.658/−-+++-
Prrc2aP(y/D)KRLKAEPA7.9/-3.711.61450/261---
Tmx3D(y/D)IIEFAHRV7.3/-4.311.67941/351---
Nfkb1G(y/D)SVLHLAI6.9/-4.611.50.26/1615+-+
Dis3l2.2I(y/D)GVVARNRA6.9/-4.711.61342/262---
UgdhL(y/D)YERIHKKML6.4/-5.211.6600/12---
Mll2S(y/D)RLPSSRKK5.9/-5.711.66673/17-+-
Galnt1L(y/D)VSKLNGP5.6/-611.627086/−---
Tpst2.2L(y/D)EAGVTDE5.5/-6.111.6−/−-+++-
Cpsf2L(y/D)DVDAAF5.3/-6.311.61410/−-++++
Zfp236.1E(y/D)LDLQTQ5.3/-6.311.61641/−-++++
Trim26.2A(y/D)ILAALTKLQ4.9/-6.711.617599/−---
Zfp236.2E(y/D)LDLQTQG4.9/-6.711.610028/−-++++-
Ube4a.2A(y/D)AKQFAA4.7/-6.911.628583/−---
Dcaf6A(y/D)RLEGDRS3.7/-7.911.6−/−---
  • a IC50 > 70,000 nM

  • Note for ELISpot results: 1–9 spots/106 CD8 = +; 10–20 spots/106 CD8 = ++; 21–50 spots/106 CD8 = +++; 51–100 spots/106 CD8 = ++++; >100 spots/106 CD8 = +++++.