Table 4.

CMS5 and Meth A epitopes with highest NetMHC PWM scores.

GeneMut/WT sequenceMut/WT ScoreMeasured IC50 for Kd (Mut/WT)ICSELISpotTumor rejection
CMS5 Epitopes with highest NetMHC scores
Ssx2ipCYAK(v/L)KEQL14.5/14.126/3.2---
Mapk1.1(q/K)YIHSANVL13.2/12.457/0.2---
FarsbHY(v/L)HIIESKPL13/13.6423/52+--
Ncoa3(h/Q)YLQYKQEDL11.5/11.72162/54074---
Mapk1.2(q/K)YIHSANV11.4/10.72135/295---
Mapk1.3LYQILRGL(q/K)YI11.3/11110/333-+++++-
Serinc1NYLLSLVAV(m/V)L11/10.22679/20861-+++++-
Meth A Epitopes with the highest NetMHC scores
Usp12SY(l/R)VVFPL14.2/12.36835/1155-+++++-
Tfdp1QYSGS(w/R)VETPV14.2/15.3-a/603---
Ufsp2HYINM(i/S)LPI14.2/14.50.23/+++b++++++-
ApcAYCETCWE(l/W)14/8.123/60++++++-
Hspg2SY(l/Q)LGSGEARL14/14.42623/79---
Ccdc85cTYIRP(f/L)ETKV13.5/13.16155/118---
Pacs2HYLS(s/A)ILRL13.4/12.741/1269---
Alms1.1(l/S)YLDSKSDTTV13.3/15.279/16+--
Alms1.2YYVPLLKRVP(l/S)13.3/7.3421/1485-++-
Ckap5K(y/D)MSMLEERI13.2/1.717/7686+--
AbrGYFVSKAKT(s/R)V13.1/12.8958/570+--
  • a IC50 > 70,000 nM

  • b +++, IC50 < 0.1 nM

  • Note for ELISpot results: 1–9 spots/106 CD8 = +; 10–20 spots/106 CD8 = ++; 21–50 spots/106 CD8 = +++; 51–100 spots/106 CD8 = ++++; >100 spots/106 CD8 = +++++.