Table 1.

Genes directly regulated by Asxl1 as determined by differential expression in HSPCs with Asxl1 deletion and directly bound by Asxl1

GeneMPsLSK cellsAnti-Asxl1 ChIP-Seq
log2 (fold change)P-valuelog2 (fold change)P-valuePeak p-valueFold enrichmentPeak FDR
Tmem87a3.919.60 × 10−63.113.68 × 10−62.78 × 10−74.210.29
Pusl11.542.21 × 10−71.301.29 × 10−33.04 × 10−85.240.15
Ddx231.451.15 × 10−101.091.79 × 10−37.45 × 10−156.930.09
Pan31.011.36 × 10−50.798.21 × 10−44.49 × 10−299.30
Srrm20.866.02 × 10−80.981.61 × 10−61.85 × 10−124.490.06
Dusp60.804.11 × 10−40.632.45 × 10−34.48 × 10−308.430
Cdca20.776.77 × 10−80.623.84 × 10−43.78 × 10−83.60.16
Apobec30.542.25 × 10−100.368.29 × 10−44.48 × 10−106.180.09
Cdc400.411.68 × 10−40.591.93 × 10−54.79 × 10−105.910.09
Rpl300.391.73 × 10−100.315.74 × 10−51.57 × 10−226.990
Rad510.393.17 × 10−30.513.48 × 10−32.28 × 10−107.160.1
Nbr10.344.65 × 10−80.430.004.04 × 10−195.690
Slc24a60.304.66 × 10−40.823.24 × 10−107.52 × 10−118.090.1
Dhrs30.284.27 × 10−30.372.16 × 10−44.33 × 10−495.150
Cdk20.231.51 × 10−40.213.94 × 10−41.06 × 10−167.890.11
Ifngr10.184.59 × 10−30.381.99 × 10−43.02 × 10−2011.890
Hsp90ab10.161.08 × 10−30.249.11 × 10−51.41 × 10−4512.710
Mtmr9−0.444.53 × 10−4−0.473.62 × 10−44.07 × 10−117.190.11
Ppp1cb−0.481.35 × 10−8−0.642.14 × 10−75.79 × 10−124.770.09
Mthfr−0.511.65 × 10−3−0.471.21 × 10−32.47 × 10−94.820.11
Ncf4−0.527.21 × 10−9−0.664.38 × 10−52.16 × 10−187.530.07
Slc25a19−0.967.68 × 10−11−0.753.50 × 10−62.24 × 10−76.640.28
Rpl29−1.180.00−0.760.002.47 × 10−95.960.11
Mettl23−1.338.43 × 10−10−1.101.21 × 10−51.10 × 10−73.680.2
Caskin2−1.543.42 × 10−5−2.451.53 × 10−44.97 × 10−135.60.07
Thbs1−1.754.71 × 10−7−1.841.63 × 10−69.53 × 10−85.060.19
  • FDR, false discovery rate. Differentially expressed genes in RNA sequencing of MPs (lineage Sca-1 c-KIT+ cells) from 1-yr-old Mx1-cre Asxl1fl/fl mice relative to Cre Asxl1fl/fl littermate controls. Differentially expressed genes in RNA sequencing of LSK cells (lineage Sca-1+ c-KIT+ cells) from 1-yr-old Mx1-cre Asxl1fl/fl mice relative to Cre Asxl1fl/fl littermate controls. Data derived from anti-Asxl1 ChIP-Seq in WT C57BL/6H BMDMs.