Table 2.

Stereotyped BCR in human PB conventional, CD5+, and CD43+ B cells

DonorsanalyzedSampleV genes obtainedV genes included in analysisaUnique sequencesbStereotyped receptorsbFisher’s exact testc
4dconventional1431251071
4dCD5+46041916012P < 0.018
2econventional1961691416
2eCD5+18015814518P < 0.018
2eCD43+11572120P < 1
  • a Only full-length V gene rearrangements and correct V-gene, i.e., VH1-69, VH3-21, VH3-23, VH3-48, and VH4-34 in stereotyped BCR-specific PCR approach

  • b Identical sequences counted as one. When all sequences obtained are counted individually, the statistical significance is below P < 0.001. Stereotypy was determined based on a 60% homology of the CDR3.

  • c P-values calculated by Fisher’s exact test versus unique conventional B cell sequences.

  • d Total numbers of VH1 and VH3 gene analysis of four independent healthy blood donors are given, for details see Table S3.

  • e Total numbers of V genes amplified by PCR for VH gene segments frequently used by stereotyped BCR of two independent healthy blood donors are given, for details see Table S7.