Table 2.

Percentages of B220loCD138+ cells after transfer into Rag1−/− mice

T cellsB cells%B220loCD138+
%
WTWT0.3 ± 0.2
Def6tr/trWT0.3 ± 0.3
WTS70-/-0.8 ± 0.3
Def6tr/trS70-/-1.5 ± 0.7
WTDef6tr/tr0.2 ± 0.4
Def6tr/trDef6tr/tr0.3 ± 0.8
WTDKO0.7 ± 0.2
Def6tr/trDKO1.5 ± 0.7
  • Percentage of B220loCD138+ cells after transfer of indicated combinations of T and B cells into Rag1−/− mice followed by immunization with SRBCs. B220loCD138+ cells were evaluated 7 d after immunization by flow cytometry. P values are as follows, WT T+WT B vs. Def6tr/tr T+WT B, NS; WT T + S70−/− B vs. Def6tr/tr T + S70−/− B, P < 0.05; WT T + Def6tr/tr B vs. Def6tr/tr T + Def6tr/tr B, NS; WT T+DKO B vs. Def6tr/tr T + DKO B, P < 0.01; WT T+WT B vs. WT T + S70−/− B cells, P < 0.01; WT T + WT B vs. WT T + Def6tr/tr B cells, NS; WT T + WT B vs. WT T+ DKO B cells, P < 0.005; Def6tr/tr T + WT B vs. Def6tr/tr T + S70−/− B cells, P < 0.05; Def6tr/tr T + WT B vs. Def6tr/tr T + Def6tr/tr B cells, NS; Def6tr/tr T + WT B vs. Def6tr/tr T + DKO B cells, P < 0.005.