Table I.

Impaired Ig secretion by STAT3MUT naive B cells in response to IL-10 and IL-21

Ig secretion
IgMIgGIgA
CD40L+IL-21+IL-10+CpGCD40L+IL-21+IL-10CD40L+IL-21+IL-10
ng/mlng/mlng/mlng/mlng/mlng/mlng/mlng/mlng/mlng/ml
Control (n = 12)30 ± 1030,646 ± 5,344640 ± 2133,223 ± 1,023<0.1538 ± 1933.8 ± 2.5<0.13,750 ± 1,440117 ± 81
STAT3MUT (n = 7)33 ± 15299 ± 16847 ± 202,382 ± 546<0.14.9 ± 1.6<0.1<0.11.8 ± 1.77.5 ± 7.5
HIES STAT3WT (n = 4)25 ± 134,125 ± 955NDND<0.1113 ± 67ND<0.1785 ± 484ND
STAT1MUT (n = 4)45 ± 4320,706 ± 7,569715 ± 5555,314 ± 3,399<0.1214 ± 114ND<0.1432 ± 275ND
  • Naive B cells from the indicated groups were cultured (10 x 103 cells/well/200 μl) with CD40L alone or together with IL-2, IL-10, or CpG. Ig secretion was measured after 10–12 d. The values are means ± SEM for the indicated number of donors with the exception of cultures of naive B cells from healthy donors or STAT3MUT patients stimulated with CD40L/IL-10 or CD40L/CpG (n = 5) and STAT1MUT patients stimulated with CD40L/IL-10 (n = 2). ND, not done.