Table I.

General and IgH locus-specific genomic instability in stimulated Polζf/Δ/CD21-cre B cells measured by FISH

General genome instabilityIgH locus-specific instability
GenotypeCytokine stimulationPercentage of IgG1+ cells on day 4Number of metaphases analyzedNumber of chromosomal aberrationsNumber of metaphases with chromosomal breaks (%)Number of metaphases analyzedNumber of metaphases with breaks in the IgH locus (%)
Polζf/+α-CD40 + IL-430.33011 (3)501 (2)
CD21-creα-CD40 + IL-436.13022 (7)502 (4)
Polζf/+/CD21-creα-CD40 + IL-428.43087 (23)501 (2)
Polζf/+/CD21-creα-CD40 + IL-426.13098 (27)501 (2)
Polζf/+/CD21-creα-CD40 + IL-432.63055 (17)501 (2)
Polζf/+/CD21-creα-CD40 + IL-434.73022 (7)501 (2)
Polζf/+/CD21-creα-CD40 + IL-423.73032 (7)500 (0)
Polζf/+/CD21-creα-CD401.0NDNDND500 (0)
Polζf/+/CD21-creα-CD400.12811 (3)500 (0)
Polζf/+/CD21-creα-RP1050.22200 (0)500 (0)
Polζf/Δ/CD21-creα-CD40 + IL-416.7304724 (80)505 (10)
Polζf/Δ/CD21-creα-CD40 + IL-413.0304120 (67)505 (10)
Polζf/Δ/CD21-creα-CD40 + IL-419.5303016 (53)503 (6)
Polζf/Δ/CD21-creα-CD40 + IL-416.6303514 (47)503 (6)
Polζf/Δ/CD21-creα-CD40 + IL-416.8301610 (33)504 (8)
Polζf/Δ/CD21-creα-CD40 + IL-418.0301911 (37)505 (10)
Polζf/Δ/CD21-creα-CD400.5301813 (43)500 (0)
Polζf/Δ/CD21-creα-CD400.120107 (35)500 (0)
Polζf/Δ/CD21-creα-CD400.12311 (4)500 (0)
Polζf/Δ/CD21-creα-RP1050.1302920 (67)500 (0)
Polζf/Δ/CD21-creα-RP1050.1303115 (50)500 (0)
  • Each row represents the results from one individual mouse.