Table III.

VH6 repertoire analysis of the splenic B cell subsets of an 8-mo-old child

DonorB cell subsetTranscriptsNumber of peaks
 analyzedaTotal number of
 sequencesNumber of different
 VDJ junctionsClonal
 diversitybP valuec
%
D3IgD+CD27+VH6 μ4685885
NaivesVH6 μ3563664
IgDCD27+
 (switched + GC)VH6 γ35012247 × 10−11
CD38+CD24
 (GC)VH6 μ355814.52 × 10−14
VH6 γ47656.510−20
  • a The peaks that were analyzed are identified by an asterisk in Fig. 2.

  • b Number of different VDJ among all sequences.

  • c Statistical significance (see Materials and methods) of differences in clonal diversity between IgD+CD27+ and IgDCD27+ or GC cells from the same donor.