Table II.

VH3.15 repertoire analysis of the splenic B cell subsets of an 8-mo-old child

DonorB cell subsetTranscriptsNumber of peaks
 analyzedaTotal number of
 sequencesNumber of different
 VDJ junctionsClonal
 diversitybP valuec
D3IgD+CD27+VH3.15 μ3585697
NaivesVH3.15 μ3716490
 (switched + GC)VH3.15 γ37335483 × 10−9
 (GC)VH3.15 μ613371534 × 10−9
VH3.15 γ598515210−8
  • a The peaks that were analyzed are identified by an asterisk in Fig. 2.

  • b Number of different VDJ among all sequences.

  • c Statistical significance (see Materials and methods) of differences in clonal diversity between IgD+CD27+ and IgDCD27+ or GC cells from the same donor.