Table I.

Affinity and kinetics measurements

hCD1d–GSL
 complexKdkoffkonkon
μMS1M1S1M1S1
α-GalCer1.29 ± 0.08a0.39 ± 0.01a3.31 × 105 ± 2.5 × 104a3.10 × 105 ± 4.0 × 104a
C20:02.73 ± 0.69a0.58 ± 0.072.38 × 105 ± 4.6 × 1042.13 × 105 ± 1.9 × 104
C20:21.83 ± 0.13a0.46 ± 0.062.46 × 105 ± 2.3 × 1042.49 × 105 ± 1.1 × 104
C11:13.40 ± 0.39a0.59 ± 0.041.65 × 105 ± 3.5 × 1041.75 × 105 ± 2.6 × 104
OCH152.50 ± 0.36a0.47 ± 0.062.04 × 105 ± 1.6 × 1041.92 × 105 ± 2.5 × 104
OCH1223.3 ± 1.41a1.00 ± 0.123.7 × 104 ± 5 × 1034.3 × 104 ± 6 × 103
OCH9123 ± 9.08a2.67 ± 0.12aND2.3 × 104 ± 1 × 103
  • All values are the mean of two experiments except where otherwise noted. The kon values were experimentally determined by SPR except those in the last column, which were calculated from the experimentally determined koff and Kd values.

  • a Mean of three or more experiments.