Table I.

Sμ DSBs in APE-deficient B cells show reduced targeting to the G:C bp and AID hotspots

nt at breakWTape1+/−ape2Y/−DBLSequencea
G73.3%b (55)79% (45)64.3% (18)67.4% (33)40.7%
C9.3% (7)1.8% (1)10.7% (3)2% (1)16.1%
A8% (6)8.8% (5)21.4% (6)14.3% (7)21.4%
T9.3% (7)10.5% (6)3.6% (1)16.3% (8)21.8%
Total75 breaks57 breaks28 breaks49 breaks2,000 nt's
G + C82.6%80.7%75%69.4%56.8%
    p-valuec0.0010.007NSNS
Hotspots48%40.4%46.4%30.6%23.2%
    p-valuec0.002NS0.047NS
  • a Frequency of nt's in the analyzed genomic sequence.

  • b Percentage of the DSBs at each nt reading the top-strand sequence.

  • c Fisher's exact t test showing the significance of difference from the Sμ sequence.