Table I.

Binding of threshold ligands to thymocytes from three different TCR transgenic mouse strains (T1, S14, and OT-1 TCR)

TCR /ligandpMHC monomerspMHC tetramers
DP thymocytes
 (Kd [M])SP thymocytes
 (Kd [M])SP thymocytes
 (r 1/2 [M])
T1/4A-Kd5.4 ± 1.6 × 10−65.5 ± 1.8 × 10−6Not done
T1/4S-Kd6.1 ± 1.1 × 10−67.9 ± 2.0 × 10−69.0 ± 1.5 × 10−8
S14/4L-Kd6.4 ± 1.5 × 10−66.2 ± 1.7 × 10−68.8 ± 1.6 × 10−8
OT-1/OvaT4-KbNot doneNot done7.6 ± 0.7 × 10−8
  • Mean values and SEMs of at least three independent experiments are shown.