Table I.

Bacterial strains

StrainRelevant genotype/phenotypeReference
E. coli
    DH5αF-endA1 hsdR17 (r− m+) supE44 thi-1 recA1 gyrA (Nalr) relA1 Δ(lacZYA-argF)U16980dLacZΔM15λpirRK6M. Swanson lab
L. monocytogenes
    10403SWT63
    DP-L2161LLO mutant64
L. pneumophila
    MB110Lp02 WT, thyA hsdR rpsL
    MB413Lp02 letA 22-3∷kan mutant (lpg2646)65
    MB416Lp02 letS 36∷kan mutant (lpg1912)65
    MB410Lp02 fliA 35∷kan mutant (lpg1782)65
    MB460Lp02 dotA∷gent mutant (lpg2646)57
    MB473Lp02 pMMBGentΔmob, vector control66
    MB552Lp02 fliD∷kan mutant (lpg1338)20
    MB553Lp02 fliD∷gent mutant20
    MB554Lp02 flhB∷gent mutant A (lpg1786)20
    MB556Lp02 flhB∷kan mutant20
    MB534Lp02 flaA∷kan mutant (lpg1340)20
    MB557Lp02 flaA∷kan mutant pMMBGent-flaA20
    MB558Lp02 flaA∷kan mutant pMMBGentΔmob vector20
    MB532Lp02 flaA∷gent mutant20
    MB559Lp02 pMMBGent-flaA20
    MB560Lp02 motAB∷gent mutant A (lpg1780-81)20
    MB562Lp02 motAB∷gent mutant A pMMB206-motAB20
    MB563Lp02 motAB∷gent mutant A pMMB206, vector control20
    MB564Lp02 fliI∷cam mutant (lpg1757)67
    MB567Lp02 flaA∷gent fliD∷kan double mutant A20
    MB569Lp02 dotA∷gent fliD∷kan double mutant A20
    MB593Lp02 dotA∷gent flaA∷kan double mutant AThis study
    MB571Lp02 motAB∷gent fliD∷kan double mutant A20
    MB314Lp01 WT prototroph, hsdR rpsL68
    MB589Lp01 flaA∷kan mutant (lpg1340)This study