Table II.

Phage-displayed peptides bind to CM22 IgM and share conserved homology with NMHC-II

PeptidesSequences
Phage clone
    P8NGNNVNGNRNNN
    ConsensusXNNNxNNxNNNN
NMHC-II
    Mouse-II A (542–556)LMKNMDPLNDNI
    human-II A (585–596)LMKNMDPLNDNI
    Mouse-II B (592–603)LMKNMDPLNDNV
    human-II B (592–603)LMKNMDPLNDNV
    Mouse-II C (607–619)LMKNMDPLNDNV
    human-II C (701–802)LMKNMDPLNDNV
ControlAGC MPY VRI PTA
  • Peptides identified by screening phage display library with IgM CM-22. Sequence of peptide region of phage clone P8 and consensus sequence based on 10 phage clones isolated from an IgM CM-22–selected pool of M13 phage display library. Alignment of conserved regions of three isoforms of mouse and human NMHC-II is also provided. The corresponding amino acid positions in each isoform are listed in the parentheses. N2 peptide corresponds to the mouse and human NMHC-II C sequence.