Table I

TLR7 and TLR9 inhibition is associated with specific sequence motifs

0.7 μM 1018 ISS1 μM R848
ODN concentrationODN concentration
1.4 μM0.35 μM0.08 μM5.6 μM1.4 μM0.35 μM
Alone100100100100100100
IRS 869: 5′-TCCTGGAGGGGTTGT-3′7123293110104
IRS 661: 5′-TGCTTGCAAGCTTGCAAGCA-3′788184214777
IRS 967: 5′-TGCTTGACAGCTTGACAGCATCCTGGAGGGGTTGT-3′128164980
IRS 957: 5′-TGCTTGACATCCTGGAGGGGTTGT-3′2410173978
IRS 954: 5′-TGCTCCTGGAGGGGTTGT-3′4719234472
IRS 986: 5′-GCTCCTGGAGGGGTTGT-3′12333885128
IRS 987: 5′-CTCCTGGAGGGGTTGT-3′1111107128142
IRS 988: 5′-AAATCCTGGAGGGGTTGT-3′332846695117
Ctrl-ODN: 5′-TCCTGCAGGTTAAGT-3′10410297108108105
  • Splenocytes from BALB/c mice were stimulated for 48 h with 0.7 μM 1018 ISS or 1 μM R848 alone or in the presence of different IRS sequences at variable concentrations as indicated. Underlined motifs are responsible for TLR7 inhibition. IL-6 production was measured by ELISA and plotted as a percentage of 1018 ISS or R848 alone. Means of four independent experiments are shown.