Table II.

ORF kinetics and function vs. T cell recognition

CD4+ T cellsCD8+ T cells
CategoryaNumber of
 HCMV ORFs
 (% of 213)Genomic
 coding spacePositive responsesb
 (n = 499)RatiocAverage positive
 response ± SEMdPositive responsesb
 (n = 378)RatiocAverage positive
 response ± SEMd
%%%
Kinetics
Immediate-early8 (3.8)4.610.4**2.30.58 ± 0.0913.8**3.02.23 ± 0.45
Early56 (26.3)29.329.51.00.85 ± 0.1123.3*0.80.55 ± 0.09
Early-late41 (19.2)22.919.20.80.73 ± 0.1520.90.90.71 ± 0.09
Late47 (22.1)22.327.9**1.30.75 ± 0.1027.5*1.21.35 ± 0.21
Function
Capsid7 (3.3)8.19.81.20.79 ± 0.209.51.20.70 ± 0.26
Matrix/tegument23 (10.8)17.027.1**1.60.74 ± 0.0920.41.21.46 ± 0.26
Glycoprotein19 (8.9)8.613.8**1.61.03 ± 0.197.40.90.50 ± 0.11
DNA/regulatory23 (10.8)18.515.80.90.50 ± 0.0724.6**1.31.42 ± 0.27
Immune evasione27 (12.7)10.520.0**1.90.82 ± 0.1118.0**1.71.25 ± 0.24
Localization
Dense bodies17 (8.0)17.027.0**1.60.88 ± 0.0922.4*1.31.16 ± 0.17
Virion60 (28.2)43.755.3**1.30.84 ± 0.0850.0*1.11.13 ± 0.13
  • a See Table SI for specific ORF assignments to each category.

  • b p-value from comparison of percentage of genomic coding space vs. percentage of positive response values: <0.05 (*) or <0.005 (**).

  • c Ratio: percentage of positive responses/percentage of genomic coding space.

  • d Percentage of peripheral blood memory compartment.

  • e ORFs implicated in immune evasion that are not essential for optimal in vitro growth (may overlap with other functional categories).