Table I.

Antigen-specific uptake of GFP-labelled pMHCI complexes from the surface of C1R target cells

HLA A*0201 heavy chain
AntigenTCRVβIFNγaWild typebD227K/T228Ab
CMV-NV96-5+4.58.9
1.73.0
27+1.31.7
0.81.3
EBV-GL920-1+9.616.4
3.32.6
25-1+4.811.2
1.73.0
  • a CD8+ T cells that do not secrete IFNγ in response to stimulation (−) represent the internal negative control for each assay.

  • b Values represent the percentage of CD8+ T cells that contain GFP for each experimental condition.