Table I.

RT-PCR primers and relative message levels for lymphoid and myeloid growth factors in the BM of naive mice

GenePrimersRelative levels mRNA in BM of naive micea
HPRTforward 5′-GCTGGTGAAAAGGACCTCT-3′1.76 ± 0.45 × 100
reverse 5′-CACAGGACTAGAACACCTGC-3′CT = 21.3 ± 0.2b
GAPDHforward 5′-AACTTTGGCATTGTGGAAGG-3′1.00 × 100
reverse 5′-ACACATTGGGGGTAGGAACA-3′CT = 22.4 ± 2.3
BAFFforward 5′-TTCCATGGCTTCTCAGCTTT-3′3.07 ± 1.00 × 101
reverse 5′-GGAATTGTTGGGCAGTGTTT-3′CT = 23.9 ± 0.4
SCFforward 5′-CGGGAATCCTGTGACTGATAA-3′6.16 ± 2.03 × 103
reverse 5′-GGCCTCTTCGGAGATTCTTT-3′CT = 29.5 ± 0.4
CXCL12forward 5′-GTCCTCTTGCTGTCCAGCTC-3′3.95 ± 1.53 × 103
reverse 5′-TAATTTCGGGTCAATGCACA-3′CT = 30.3 ± 4.0
IL-1βforward 5′-GACCTTCCAGGATGAGGACA-3′8.74 ± 0.28 × 104
reverse 5′-AGGCCACAGGTATTTTGTCG-3′CT = 32.6 ± 0.2
IL-7forward 5′-TGGAATTCCTCCACTGATCC-3′4.93 ± 1.75 × 104
reverse 5′-ACCAGTGTTTGTGTGCCTTG-3′CT = 33.1 ± 0.3
CXCL2forward 5′-AGTGAACTGCGCTGTCAATG-3′1.19 ± 0.17 × 104
reverse 5′-AGGCACATCAGGTACGATCC-3′CT = 36.1 ± 0.7
IL-3forward 5′-TAGGGAAGCTCCCAGAACCT-3′<1.00 × 105
reverse 5′-TTAGGAGAGACGGAGCCAGA-3′NA
GM-CSFforward 5′-CATCAAAGAAGCCCTGAACC-3′<1.00 × 105
reverse 5′-TGCATTCAAAGGGGATATCAG-3′NA
  • a mRNA levels are standardized to GAPDH message; levels of a second housekeeping gene, HPRT, control for nonspecific changes in message levels.

  • b CT values represent average threshold cycle number (±SD) for amplifications of cDNA reverse transcribed from 0.025 μg total RNA.

  • NA, not applicable.