Table III.

Frequency of GFP+ cells in the brains of transplanted or parabiotic mice expressing neural lineage markers

No. GFP+ neurons
Engraftment
 modelAnimal
 no.Age at
 transplant or
 parabiosisNo. of cells
 transplantedDuration of
 survival after
 transplant/
 parabiosisInjury
 modelBlood chimerism
 at death
 (% GFP+ PB cells)No. GFP+
 Purkinje cells
 (calbindin+ GFP+
 mormorphologically
 identified cells/
 total GFP+ cells)Markers
 evaluatedNo. of
 qualifying
 cells/
 total GFP+
 cellsNo. GFP+
 astrocytesa(GFP+GFAP+/
 total GFP+ cells)
WBM
   transplant1newborn1069 mono injury131/1,099NeuN+, CD450/4120/209
2newborn1069 mono injury55/1,287NeuN+, CD450/4510/182
3adult1063 mono injury880/600NeuN+, CD45,
 Mac0/2910/310
4adult1063 mono injury630/680NeuN+, CD45,
 Mac0/3850/295
5adult1066.5 moKA920/429NeuN+, CD45,
 Mac10/1980/231
6adult1067 moKA940/1,065NeuN+, CD45-0/1,0100/55
HSC
   transplant8adult13 mono injury720/538NeuN+, CD45-,
 Mac10/1050/65
19adult1005 morunning980/259NeuN+, CD450/259ND
20adult1005 morunning980/161NeuN+, CD450/161ND
9adult14.5 moKA270/183NeuN+, CD450/420/51
10badult19 moKA980/710NeuN+, CD450/6550/55
Parabiosis21cadultNA7 mono injury460/16NeuN+, CD450/140/2
17adultNA3 moKA410/79NeuN+, CD450/620/17
  • a The number of GFP+ cells per section evaluated in the GFAP study was estimated from the average number of GFP+ cells in several representative sections from the same animal.

  • b This animal received 2 × 106 unfractionated BM cells from an animal previously transplanted with a single HSC.

  • c Animal 21 was injected intramuscularly with cardiotoxin 8 wk before being killed, which has no known effects on the brain.

  • NA, not applicable.