Table I.

Analysis of microglial marker expression by GFP+ cells in brains of transplanted or parabiotic mice

Engraftment
 modelAnimal
 no.Age at
 transplant
 or parabiosisNo. of cells
 transplantedDuration of
 survival after
 transplant/parabiosisInjury
 modelHematopoietic
 chimerism
 at death
 (% GFP+
 PB leukocytes)Iba1+ donor cells
 per section, mean ± SD
 (total cells analyzed:
 GFP+Iba+/GFP+)GFP+ microglia
 per section, mean ± SD
 (total cells analyzed:
 GFP+Iba+/Iba+)
%%
WBM
   transplant1newborn1069 mono injury1378.8 ± 2.2
 (167/212, n = 2)2.2 ± 0.8
 (167/7,607, n = 2)
2newborn1069 mono injury580.6 ± 7.0
 (241/299, n = 3)4.3 ± 2.3
 (241/5,613, n = 3)
3adult1063 mono injury8892.3 ± 2.7
 (886/960, n = 3)11.5 ± 1.7
 (886/7,697, n = 3)
4adult1063 mono injury6385.0 ± 2.3
 (597/703, n = 2)6.0 ± 0.5
 (597/9,890, n = 2)
5adult1066.5 moKA9290.3 ± 5.5
 (475/526, n = 2)4.8 ± 1.8
 (475/9,856, n = 2)
6adult1067 moKA9458.0 ± 19.2
 (409/705, n = 2)4.9 ± 0.6
 (409/8,393, n = 2)
HSC
   transplant7adult13 mono injury1093.1 ± 5.5
 (54/58, n = 4)0.5 ± 0.1
 (54/10,865, n = 4)
8adult13 mono injury7253.7 ± 23.8
 (36/67, n = 3)0.8 ± 0.03
 (36/4,363, n = 3)
9adult14.5 moKA2797.6 ± 4.7
 (82/84, n = 2)1.5 ± 0.7
 (82/6,939, n = 2)
10aadult19 moKA9891.0 ± 3.3
 (343/377, n = 2)7.2 ± 2.1
 (343/4,755, n = 2)
11adult1004 moKA8899.3 ± 0.8
 (279/281, n = 2)4.7 ± 1.1
 (279/5,598, n = 2)
12adult1007 moKA4758.1 ± 14.4
 (864/1,488, n = 2)10.7 ± 0.4
 (864/8,230, n = 2)
Parabiosis13adultNA7 mono injury2242.8 ± 23.5
 (62/145, n = 3)1.4 ± 0.2
 (62/3,302, n = 3)
16adultNA9 dno injury60.200
14adultNA9 dKA5233.3 ± 70.7
 (1/3, n = 2)0.01 ± 0.1
 (1/7,380, n = 3)
15adultNA16 dKA6116.7 ± 11.6
 (2/12, n = 3)0.02 ± 0.03
 (2/11,330, n = 4)
17adultNA3 moKA4120 ± 44.9
 (3/15, n = 4)0.04 ± 0.03
 (3/7,733, n = 4)
  • a This animal received 2 × 106 unfractionated BM cells from a single HSC transplanted mouse.

  • NA, not applicable.