Table II.

HSC Activity in GATA-2 Mutant Tissues after Explant Culture

AGM explantsYS explantsFL explants
Stage +/++/−+/++/−+/++/−
E10.52a/3b (66)c1/12 (8)0/3 (0)0/11 (0)ND0/2 (0)
E11.01/2 (50)0/4 (0)1/4 (25)1/5 (20)0/3 (0)0/5 (0)
E11.511/12 (50)0/27 (0)7/25 (28)7/29 (24)6/25 (24)3/28 (11)
E12.07/12 (58)0/13 (0)4/16 (25)2/14 (14)11/12 (92)10/19 (53)
+/++/−−/−+/++/−−/−+/++/−
E10.00/7 (0)0/8 (0)0/3 (0)0/7 (0)0/8 (0)0/3 (0)NDND
  • a the number of recipient mice showing donor cells in the peripheral blood (DNA) isolated at >4 mo posttransplantation,

  • b the total number of mice transplanted, and

  • c the percentage of repopulated recipients.

  • ND, transplantation not performed. Only when donor contribution was >10% was the recipient considered to be positive. Two to four independent experiments performed for each stage and tissue type. For E10, one to five tissue explant equivalents were transplanted per recipient. 1 tissue explant equivalent was transplanted per recipient for E10.5 and 0.33 tissue explant equivalents for E11.0, E11.5, and E12.0. +/+, GATA-2+/+ and +/−, GATA-2+/−. E10, 31–35 somite pairs (sp); E10.5, 36–40 sp; E11.0, 41–47 sp; E11.5 > 48 sp; E12.0 > 60 sp.

  • E10 to E12 AGM, YS, and FL explants were cultured for 3 d, made into a single cell suspension, and injected into irradiated adult recipients. Each result represents