Table IV.

Somatic Hypermutation of the JH2-JH4 Region from Peyer's Patch B Cells

A.Msh3 controlMsh6 control
AGCTTotalAGCTTotal
A/1651233A/95519
G9/4114G10/3316
C02/79C32/1217
T435/12T436/13
6865
Msh3−/−Msh6−/−
AGCTTotalAGCTTotal
A/35132068A/0112
G19/4629G10/2214
C37/919C21/2225
T141019/43T200/2
15943
B.ControlsMsh6−/−Msh3−/−
GC mutations/total56/133 (42%)39/43 (91%)a48/159 (30%)c
Tsb mutations at GC/total38/56 (68%)32/39 (82%)d28/48 (58%)e
Hotspot mutations/total25/133 (11%)15/43 (35%)f23/159 (14%)g
  • a, f Statistically significant difference between Msh6−/− and control mice (χ2 test; aP < 0.0001, fP = 0.0478).

  • b Ts, transition.

  • c Statistically significant difference between Msh3−/− and control mice (χ2 test; P = 0.0218).

  • d No statistically significant difference between Msh6−/− and control mice.

  • e, g No statistically significant difference between Msh3−/− and control mice.