Table I.

MPI Compounds from Bacteria and Eukaryotic Cells

T cell
 stimulationbFragment ions (MS/MS)e
Speciesa[M-H]c(normalized
 intensity)dLipid
 length[M-H2O-H][M-C4H8O4-H]Alkyl
 phosphate ionHexosyl
 phosphate ion
M. tuberculosis+679 (10.7)C30661559517241
H37Rv693 (3.9)C31675573531
707 (100)C32689587545241
721 (2.4)C33703601559
735 (5.9)C34717615573241
M. tuberculosis+679 (100)C30661559517241
CDC1551707 (5.7)C32689587545241
M. bovis BCG+679 (10.6)C30
Pasteur693 (6.1)C31
707 (100)C32689587545241
721 (5.6)C33
735 (5.0)C34
M. avium+679 (100)C30661559517
693 (0.2)C31
707 (0.8)C32689587545
721 (0.8)C33
M. tuberculosis+707fC32
H37Ra
Human
   mononuclear cells
  • a Total lipid extracts from M. smegmatis, M. phlei, M. fallax, R. equi, N. farcinica, S. aureus, E. coli, S. pombe, C. albicans, A. niger, L. donovani, and L. tropicana did not stimulate T cells when tested as in Fig. 1 B.

  • b The CDIc-mediated T cell activity in the crude lipid extracts were considered as (+) when greater than twofold proliferation of T cells was seen in response to total lipid fractions tested as in Fig. 1 B.

  • c [M-H] ions of MPI compound detected by LC-MS analyses.

  • d Intensity of each MPI compound when the most abundant MS signal from each preparation is 100.

  • e Detected fragment ions by low energy CDI-MS analysis for a listed parent ion (left).

  • f See reference 11.