Table I.

Copy Number of Selected var Genes and seryl-tRNA synthetase Transcripts in Unselected and VSAUM-expressing 3D7 and 3D7 after Antibody Selection for VSASM Expression

Trophozoite/schizont-stage parasites
 (36–48 h after invasion)Ring-stage parasites
 (30 h after invasion)
ClusteraPrimerbGeneUnselectedAntibody-selectedFold changeUnselectedAntibody-selectedFold change
CopiesCVcCopiesCVcCopiesCVcCopiesCVc
A8PF11_0521426117,1192716.712,5703184,341296.7
20PF13_00031,79776,129203.477,93413179,74692.3
35PFD1235w/MAL7P1.12,9312029,9411310.2101,417271,152,7581211.4
67MAL6P1.3143,1451319,356146.287,13019767,74788.8
97PFA0015c77,890121,3333058.4155,1512118,419148.4
B/A94MAL6P1.31613,7421536,637242.7247,156291,057,056284.3
B18PFL0005w15211,1062373.79281213,7692314.8
21PFB1055c64162,5331239.65,2722226,07054.9
58PFI1830c79,1113959,860221.318,6001513,124121.4
B/C12PFL1950w2262252482.313,2361919,053241.4
C66PF08_010782,211335,5901516.3993,6792358,1301717.1
95PFD0995c23,415194911247.7567,205199,1481962
var210PFL0030c9071412,562191412,90530127,631309.9
var137PFE1640w12,2792114,239101.28,224179,111301.1
seryl-tRNA synthetase609,53316609,533161643,27120643,271201
  • a Described in reference 18.

  • b Described in reference 32 and Table S1.

  • c Coefficient of variation (%) between three different quantification experiments.