Table III.

Proliferation and Cytokine Production by HIV Peptide–specific CD4+ T Cells in Viremic Patients

PatientPeptideSequencePercent
 CFSElow CD4+DBLFaCD69+ IL-2+CD69+ IFN-γ1IL-2+/IFN-γ1IL-2+IFN-γ1
111G054DRVHPVHAGPIAPGQ0.020.0008<0.010.40<0.01<0.010.35
G067WIILGLNKIVRMYSP0.10.0005<0.010.12<0.01<0.010.15
112G008YKLKHIVWASRELER0.30.001<0.010.10<0.01<0.010.15
G067WIILGLNKIVRMYSP0.10.0005<0.010.10<0.01<0.010.17
113G065PVGEIYKRWIILGLN0.50.002<0.010.30<0.01<0.010.35
G067WIILGLNKIVRMYSP0.30.001<0.010.40<0.01<0.010.46
114G053AAEWDRVHPVHAGPI0.50.002<0.010.10<0.01<0.010.19
G076YKTLRAEQASQEVKN0.40.002<0.010.10<0.01<0.010.17
115G067WIILGLNKIVRMYSP0.40.002<0.010.20<0.01<0.010.21
G061STLQEQIGWMTNNPP0.20.0008<0.010.20<0.01<0.010.26
116N048LAFHHVARELHPEYF0.350.001<0.010.12<0.01<0.010.15
G065PVGEIYKRWIILGLN0.30.001<0.010.11<0.01<0.010.17
117G004LDRWEKIRLPRGGKK0.50.002<0.010.42<0.01<0.010.40
N036WCYKLVPVEPDKVEE0.40.002<0.010.31<0.01<0.010.30
118N022VDLSHFLKEKGGLEG0.40.002<0.010.20<0.01<0.010.23
G057PGQMREPRGSDIAGT0.20.0008<0.010.23<0.01<0.010.24
Median0.3210.001NA0.21NANA0.24
  • a DBLF, deduced baseline frequency derived from the 6-d CFSE-based proliferation assay.

  • Values of percent CFSElow CD4+ are the medians of five independent experiments. The percentages of cytokine production were measured after 12 h after stimulation of patients' PBMCs with HIV-specific peptides. The data depicted are the medians of three separate experiments. Values <0.01 indicate undetectable frequencies. NA, not applicable.