Table II.

Proliferation and Cytokine Production by HIV Peptide–specific CD4+ T Cells in Aviremic Patients

PatientPeptideSequencePercentCFSElow CD4+DBLFaCD69+ IL-2+CD69+ IFN-γ1IL-2+/IFN-γ1IL-2+IFN-γ1
12CG067WIILGLNKIVRMYSP200.110.10.050.050.05<0.01
G065PVGEIYKRWIILGLN150.080.070.030.030.04<0.01
12BG076YKTLRAEQASQEVKN80.050.050.020.020.030.03
G067VDLSHFLKEKGGLEG60.050.050.020.020.03<0.01
11CG065IYKRWIILGLNKIVR80.070.070.030.030.04<0.01
G067WIILGLNKIVRMYSP7.50.040.050.020.020.03<0.01
13CG067DRVHPVHAGPIAPGQ70.030.050.020.020.03<0.01
N010LEKHGAITSSNTAAT6.50.030.040.020.020.02<0.01
10CN012SSNTAATNAACAWLE7.50.050.040.020.020.02<0.01
G072IRQGPKEPFRDYVDR60.030.040.020.020.02<0.01
09BG057PGQMREPRGSDIAGT100.050.060.030.030.03<0.01
G040KVVEEKAFSPEVIPM70.030.050.020.020.03<0.01
02BN010LEKHGAITSSNTAAT90.050.060.030.030.03<0.01
G055PVHGPIAPGQMREP7.50.040.040.020.020.02<0.01
03CN048LAFHHVARELHPEYF110.060.070.040.040.03<0.01
G040KVVEEKAFSPEVIPM6.30.040.040.020.020.02<0.01
11BG065IYKRWIILGLNKIVR150.080.080.040.040.04<0.01
N012SSNTAATNAACAWLE80.040.050.020.020.03<0.01
07BN017EVGFPVIPQVPLRPM120.060.070.040.040.03<0.01
G057PGQMREPRGSDIAGT6.70.030.040.020.020.02<0.01
Median9.20.0510.0560.0260.0260.029NA
  • a DBLF, deduced baseline frequency derived from the 6-d CFSE-based proliferation assay.

  • Values of percent CFSElow CD4+ are the medians of five independent experiments. The percentages of cytokine production were measured after 12 h after stimulations of patients' PBMCs with HIV-specific peptides. The data depicted are the medians of three separate experiments. Values <0.01 indicate undetectable frequencies. NA, not applicable.