Table I.

B Lymphocyte Populations in Vav-null Mice

TotalNewly formedT1T2FollicularMZ
Wild type94.5 ± 38.34.5 ± 1.45.3 ± 2.96.3 ± 2.012.9 ± 8.61.9 ± 1.3
Vav null88.7 ± 7.620.6 ± 3.915.6 ± 3.52.5 ± 1.10.7 ± 0.30.5 ± 0.1
  • Cells from spleens of WT and Vav1/2/3ko (Vav-null) mice were counted and stained for cell surface expression of B220, IgM, IgD, CD23, and CD21/35, as shown in Fig. 3 A, and analyzed by flow cytometry. Numbers of indicated populations are shown for newly formed (NF or B220+CD21CD23), transitional 1 (T1 or B220+CD21loIgMhiIgDlo), transitional 2 (T2 or B220+CD21hiIgMhiIgDhi), follicular (FO or B220+CD21intCD23hiIgMloIgDhi), and marginal zone (MZ or B220+CD21hiCD23lo) cells. Data are presented as means ± SDs and represent multiples of 106 cells (n = 3). Total cells were counted by gating live lymphoid cells.