Table I.

Composition of Mucosal Lymphoid Populations in H. hepaticus–infected RAG−/− Mice

TissueGroupCell no.NeutsMonosDCNKCD4+
 T
%%%%%
LPLControl2.8 × 1040.618.28.83.00.3
Hh2.1 × 10517.513.06.08.10.3
Hh + 25+2.5 × 1040.414.67.35.815.2
MLNControl1.4 × 1051.815.14.547.70.2
Hh3.6 × 1054.031.74.725.20.3
Hh + 25+7.7 × 1050.510.25.615.822.1
  • Groups of 129SvEvRAG2−/− mice were left untreated (Control), or infected by oral gavage with 2 × 108 cfu H. hepaticus (Hh), or infected with H. hepaticus and reconstituted with 1 × 105 CD4+ CD45RBlowCD25+ T cells (Hh + 25+). Mice were sacrificed 8–12 wk later and LPL and MLN populations (pooled from 4–6 mice per group) were characterized using FACS® analyses. Mean cell number for each tissue was calculated by dividing the total number of cells isolated by the number of mice pooled. Results shown are representative of two similar experiments.