Table I.

VCAM-1 Is Expressed on the Endothelial Luminal Surface of Almost all HEVs in BALT and on Rare HEVs in Other Secondary Lymphoid Tissues

NODBALB/c
TissueAddressin onlyaVCAM-1 onlyDual expressionAddressin onlyaVCAM-1 onlyDual expression
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BALT6.2 ± 1.12.6 ± 1.291.2 ± 1.9b6.4 ± 1.23.0 ± 1.790.6 ± 2.0b
PLN94.6 ± 1.80.4 ± 0.25.0 ± 1.895.4 ± 2.30.6 ± 0.44.0 ± 2.1
MLN94.8 ± 2.91.0 ± 0.64.3 ± 2.796.2 ± 3.30.2 ± 0.23.6 ± 3.1
PP98.3 ± 0.60.0 ± 0.01.8 ± 0.674.2 ± 17.60.8 ± 0.89.0 ± 3.0
  • a Addressin = PNAd for BALT, PLN and MLN; MAdCAM-1 for PP.

  • b In each strain, the percentage of BALT HEVs with dual expression was significantly greater than the percent of PLN, MLN, and PP HEVs with dual expression (P < 0.001, unpaired t test). There were no differences between the two strains in the patterns of adhesion molecule expression by BALT HEVs.

  • Five NOD and five BALB/c mice were injected intravenously with anti–VCAM-1 mAb. The numbers of HEV that expressed addressin only, luminal VCAM-1 only, or both were determined by tissue section IF staining and confocal or IF microscopy. Results are expressed as mean ± SE. Mean number of HEV examined in each tissue in each mouse: BALT = 29, PLN = 182, MLN = 72, and PP = 41.