Table I.

Diabetes Incidence in InsHA Mice

Islet infiltrationa
Adoptive transfer and treatmentClearPeriinsulitisInsulitisIslet destructionDiabetes incidenceb
(%)(%)(%)(%)(%)
Clone 4n = 33100000n = 210
Clone 4 plus HNTcn = 2793700n = 110
Clone 4 CD8+ plus activated DO11n = 18891100n = 60
Clone 4 CD8+ plus activated HNTn = 3001090100n = 988
Clone 4 plus activated HNT plus anti-B7.1
 plus B7.2n = 234444813n = 60
Clone 4 plus IL-2cn = 2296400n = 80
Clone 4 plus anti-CD40cn = 3197300n = 110
Clone 4 plus anti-CD40 plus IL-2cn = 2496400n = 90
Clone 4 plus anti-CD40 plus IL-12n = 36455500ND
Activated HNTcn = 268771514n = 90
HNTcNDNDNDNDn = 60
  • a Total numbers of islets examined in each group is indicated. Pancreatic sections from two to four mice per group were analyzed, except in the group that received clone 4 plus anti-CD40 plus IL-12 in which sections from seven mice were studied.

  • b Total number of mice monitored for diabetes per group is indicated.

  • c Conditions for which a group of three mice was monitored for 20 d.

  • Groups of InsHA mice were treated as indicated and monitored for diabetes for 8 or 20 d. Mice were considered diabetic when the blood glucose levels were >300 mg/dl. Pancreas from mice sacrificed on day 8 were subject to histological analysis to determine the presence of islet infiltrates. Islet destruction was determined by the absence of insulin staining. ND, nondetermined.