Table III.

Exposure to SDF-1α Does Not Alter the Long-Term Engraftment Potential of LT-HSC Injected at Near Limit Dilution Doses

Hours of exposureReconstitution pattern
nIn cultureSDF-1αLTMRPercent
 LTMRB, TB, MBNone
A
6004670002
6304672000
530.53602000
6314671001
8324503100
B
8304501012
7323431003
  • (A) Experiment 1. The long term competitive repopulation assay was performed (Materials and Methods) with 40 double-sorted LT-HSC injected per recipient mouse. LT-HSC were incubated in vitro without (in culture) or with SDF-1α as indicated. Peripheral blood of recipient mice was analyzed by flow cytometry for the presence of donor-derived B, T, and myeloid cells 31 wk after transplant. Data shown are the number or percentage of mice with the indicated reconstitution patterns. An RxC test of independence using the G test with the Williams' correction revealed that there are no significant differences among the groups (0.9 < P < 0.975). (B) Experiment 2. Same as experiment 1, except that 30 LT-HSC per recipient were injected and the mice were analyzed 17 wk after transplant. An RxC test of independence using the G test with the Williams' correction revealed that there are no significant differences between the groups (0.5 < P < 0.9). Abbreviations: B, B cells, LTMR, long-term multilineage reconstitution; M, myeloid cells; T, T cells.