Table II.

Adoptively Transferred Tumor-specific T Cells Retain Functionality in RIP(GP × Tag2) Hosts

PDLN cellsSplenocytes
Recipient genotypeAdoptive transfer of P14 T cellsMB6 targetsAV targetsMB6 targetsAV targets
RIP (GP × Tag2)+202452
RIP (GP × Tag2)+182352
  • 5 × 106 P14 TCR transgenic T cells were adoptively transferred into the indicated recipient mice. 6 wk after transfer, all mice were infected with 2,000 PFU LCMV intravenously. 8 d after infection, PDLN cells and splenocytes were assayed for their cytotoxic activity against EL-4 targets pulsed with MB6 or negative control AV peptide. Data is expressed as the percentage of specific lysis at effector/target ratios of 60:1, with 104 effectors from spleens plated per well and only 5,000 effectors from draining LNs plated per well, due to the limited number of cells available in draining LNs. Spontaneous 51Cr release was <15%. Results are representative of data from two independent experiments.