Table II.

In Vitro Production of Cytokines in Spleen Cells from Control and Vaccinated Balb-neuT mice after Restimulation with HER-2/neu-positive (Neu/H-2q) and -negative (Neuneg/H-2q) Allogeneic Cells or with HER-2/neu-positive Syngeneic Cells (Neu/H-2d)

Released cytokine (ng/ml)
Vaccinated miceaIn vitro stimulationIFN-γIL-4
MSANone0.12 ± 0.070.00 ± 0.00
Neu/H-2q0.27 ± 0.070.00 ± 0.00
Neuneg/H-2q0.40 ± 0.230.00 ± 0.00
Neu/H-2d0.35 ± 0.110.00 ± 0.00
Neu/H-2q plus
 IL-12None0.65 ± 0.120.04 ± 0.03
Neu/H-2q3.37 ± 0.95b0.18 ± 0.11
Neuneg/H-2q2.86 ± 0.64b0.10 ± 0.05
Neu/H-2d1.81 ± 0.320.11 ± 0.00
  • a Mice received two courses of vaccination.

  • b P < 0.05 versus no restimulation (Student's t test).