Table I.

Reactive Cell Content, Expression of Endothelial Adhesion Molecules, and Production of Cytokines, Angiogenic Factors, and Mediators in Tumors Growing in Balb-neuT Mice

MSAIL-12Neu/H-2q cellsplus IL-12
Reactive cellsa
Dendritic cells1.4 ± 0.711.0 ± 3.2b9.3 ± 2.8b
Macrophages3.1 ± 1.34.2 ± 1.822.1 ± 4.2c
PMN4.5 ± 2.04.6 ± 2.323.3 ± 5.2c
CD8+ lymphocytes1.3 ± 0.96.7 ± 2.4b12.7 ± 3.7c
CD4+ lymphocytes0.01.3 ± 0.4b2.0 ± 0.6
NK cells5.1 ± 1.914.6 ± 3.3b11.2 ± 3.5b
Microvessel count14.4 ± 2.29.0 ± 1.8b6.1 ± 1.7b
Endothelial adhesion moleculesd
ELAM-1+
ICAM-1++++
VCAM-1±
Cytokines and
 mediatorsd
IL-1β+++
TNF-α± ±+
IFN-γ ±+
MCP-1+++
MIP-2±±+
RANTES±
IP-10+++++
MIG+++++
iNOS++
Angiogenic factorsd
VEGF±±±
bFGF+++
  • a Cell counts performed in ten randomly chosen high-power fields per sample. Results are mean ± SD of positive cell per field.

  • b Significantly different (P < 0.001) from corresponding values in MSA control mice.

  • c Significantly different (P < 0.001) from corresponding values in MSA control and IL-12–treated mice.

  • d The expression of adhesion molecules, cytokines, mediators, and angiogenic factors was defined as absent (-), scarcely (±), moderately (+), and strongly (++) present on cryostat sections decorated with the antibody.

  • bFGF, basic fibroblast growth factor; ELAM, endothelial leukocyte adhesion molecule E-selectin; ICAM, intercellular adhesion molecule; iNOS, inducible nitric oxide synthase; MCP, monocyte chemotactic protein; VCAM, vascular cell adhesion molecule; VEGF, vascular endothelial cell growth factor.