Table 1

TREM-2–dependent Regulation of Cell Surface Activation Markers

F(ab′)2 anti–TREM-1F(ab′)2 anti–TREM-2LPS
TREM-2112.233.457.1
MHC class I67.865.3107.1
MHC class II119.12278.65454.67
CD40171.35598.6635.89
CD8032.139.4104.6
CD86/B7.214.04387.91683.56
CCR512.9513.563.12
CCR63.683.454.01
CCR76.8221.9812.45
CXCR45.134.5617.8
CD11a10.926.7813.72
CD11b53.965.723.1
CD11c91.165.7123.5
CD2938.2237.5637.5
CD414.544.674.39
CD54/ICAM-156.8754.78271.45
CD614.955.034.21
CD1033.633.963.26
Mannose-R81.882.930.9
CD64/FcγR I9.810.12.3
CD32/FcγR II17.2116.782.34
CD89/FcαR4.544.754.96
CD35/CR 13.944.233.67
M-CSF-R14.64.235.21
GM-CSF-R15.613.713.5
CD382.52.243.5
CD833.343.2326.7
CD1a106.76134.987.54
  • DCs were cultured for 48 h in plates coated with control F(ab′)2, F(ab′)2 anti–TREM-2 mAb, or LPS as indicated in Fig. 6. Cells were subsequently analyzed by flow cytometry for the indicated cell surface molecules. Numerical values indicate specific mean fluorescence intensity after subtraction of the fluorescence detected with an isotype-matched control. The data shown are representative of four independent experiments.