Table 3

Formation of C3 Fragments in Blood after Vascular Stasis Requires the Classical Complement Pathway and Plasma Ig

Vascular stasis time
Mouse strain15 min30 min
Normal100 ± 22100 ± 11
Clqa−/5 ± 24 ± 2
C4−/0 ± 00 ± 0
Bf−/46 ± 3319 ± 2
H2-Bf,C2−/0 ± 00 ± 0
RAG−/13 ± 24 ± 1
  • Blood was collected from normal C57BL/6, complement component–deficient, and Ig-deficient (RAG−/−) mice, as in Fig. 4, and the level of C3 fragments generated 15 and 30 min after death determined by immunofluorescence flow cytometry, as illustrated in Fig. 1 A. Results are expressed as the percentage of C3 fragments present compared with the level generated in blood from normal C57BL/6 mice. All results are presented as means ± SD, and data are representative of at least two separate experiments.