Table 1

Intestinal Epithelia Resist Hypoxia-elicited Disruption

Paracellular flux
Cell typeNormoxiaHypoxiaFold increaseP value
pM/cm2/hpM/cm2/h
Epithelia (T84, colon)5 ± 0.45 ± 0.30.0NS
Epithelia (Caco-2, colon)8 ± 1.69 ± 0.80.1NS
Epithelia (KB, oral)97 ± 8.1430 ± 12.64.4<0.001
Epithelia (MDCK, kidney)63 ± 10.4245 ± 12.23.8<0.001
Epithelia (A549, lung)197 ± 19.5482 ± 52.52.4<0.007
Epithelia (MVEC, dermis)127 ± 5.1395 ± 14.63.1<0.001
  • Monolayers of indicated cell type were grown to confluence on semipermeable supports. Monolayers were exposed to normoxia (pO2 = 147 Torr) or hypoxia (pO2 = Torr) for 24 h (48 for A549 cells) at 37°C. Paracellular permeability of fluoresceinated dextran (40 kD, 70 kD for A549 cells) was assessed over a 60-min period and flux was calculated from a standard curve. Results represent mean and SEM from at least three separate experiments.