Table 1

Analysis of Lymphocyte Subsets in Cockroach Allergen-challenged Lungs of Control and CCR8−/− Mice

GroupTotal cells (107)CD4+ (106)CD8+ (106)CD19+ (106)
CCR8+/+2.2 ± 0.161.2 ± 0.80.50 ± 0.20.8 ± 0.2
CCR8−/1.6 ± 0.181.8 ± 0.140.58 ± 0.31.3 ± 0.14
P0.015>0.05>0.05>0.05
  • Cockroach antigen sensitization and challenge was performed as described in Materials and Methods. Lungs were collected and dispersed 24 h after the final intratracheal challenge, and then subjected to flow cytometric analysis. Values are means ± SD derived from four individual mice. The reduction in total cell numbers in CCR8−/− was due to decreased eosinophil recruitment (CCR8+/+, 25.5 ± 5% versus CCR8−/−, 14 ± 4%). Control and knockout mice were on inbred 129 background.