Table 4

Primers Used for Construction of Recombinant Human C4bp Deletion Mutant Molecules

rC4bp proteinAmino acids deletedSense primer (5′→3′)
rC4bp ΔSCR1N1 to Y62CCT GCT GTT CTT GGC AAA CGA TGC AGA CAC
rC4bp ΔSCR2K63 to I124ACC TTC TGT ATC TAC GTC AAG TGT AAG CCT
rC4bp ΔSCR3V125 to K188CCA CAA TGT GAA ATT ATC ACC TGT CGC AAG
rC4bp ΔSCR4K188 to N249CCT CCT ACC TGT GAA AAT AGT TGT ATT AAT
rC4bp ΔSCR5N 249 to A314CCT GCT TGT GAG CCC TTA TGT TGC CCT GAA
rC4bp ΔSCR6A314 to G375TAC CAA GGA TGT GAG GAC ATT TGC AAT TTT
rC4bp ΔSCR7D376 to K433ACA CCA TCA TGT GGA GCT CTG TGC CGG AAA