Table 1

pH of Young and Mature Vacuoles

ConditionAgenpHError
Phagosomes containing NHS-carboxyfluorescein–labeled particlesLive E. coli45–75 min175.60.32
Live L. pneumophila3–6 h567.40.9
Heat-killed L. pneumophila2–4 h415.50.3
Vacuoles containing fluorescein dextranL. pneumophila16–22 h645.60.8
L. pneumophila + 1 μM BFA17–20 h227.30.8
Macrophage vesicles2 h3005.50.3
Macrophage vesicles + 1 μM BFA2 h907.60.59