Table 1

ES Cell Viability after Adriamycin-induced Apoptosis

Viability
Adriamycinp38α+/+p38α+/+ + SB-203,580p38α−/p38α−/− + SB-203,580
μg/ml%%%%
072646866
0.0551484336
0.1644373029
0.5028272221
1.5021191724
4.5023201719
  • ES cells (2 × 106 cells per well in 6-well cluster plates) were incubated for 18 h with the indicated concentration of adriamycin. Where indicated, SB-203,580 was also present at 10 μM. The cells were subsequently harvested and analyzed by flow cytometry after staining with FITC-labeled annexin V and propidium iodide. The percentage of viable cells (those not staining with either annexin V or propidium iodide) was determined based on 10,000 gated events. Data are representative of three separate experiments.