Table 1

Cytogenetic Analysis of NFATp−/− Cartilage Cell Lines

TumorKaryotype
140,XY
240–41,XX[cp7]/39–40,xx-4[6],-12[7],+17[2], +mar[5][cp7]/43,X,der(?X),add(1),der(?6),+?7, +9,+mar5[3]
340,X-X,+mar[3]/39,X-X,-14,+mar[2]/41, XX+mar[4]/40,XX[41]
471–89,XXXX-2[6],-3[10],-4[12],-4[10],-6[2], -7[8],-9[6],-10[2],-11[4],-12[3],-13[2],-14[3], -15[2],-16[2],-17[2],+18[4],+1-6mar[9][cp12]
  • Aneuploidy in NFATp−/− tumor cells. Karyotypic analysis of four tumor cell lines from NFATp−/− mice revealed aneuploidy in three of them. The first line was a normal male with random chromosome loss. The second line had a modal chromosome no. of 40 in 7 cells with monosomy of chromosomes 4 and 12, trisomy of chromosome 17, and gain of markers in most of the cells. An additional three cells displayed additional structures and numerical abnormalities. 2 clones were observed in the third tumor, a normal 40XX clone in 41 cells, and an abnormal clone that demonstrated clonal evolution. A marker chromosome was observed in all cells in the clone, and in addition, five cells demonstrated loss of an X and two of these also had loss of chromosome 14. The fourth tumor, derived from extraarticular cartilage, was pseudotetrapoid with a modal chromosome no. of 71–76 (range 71–89), with a consistent finding of loss of chromosomes 4 and 16. One to six marker chromosomes were observed in most cells, and there were no normal diploid or tetraploid cells. mar, marker; der, derivative; add, additional material of unidentified origin.