Table 1

Amino Acid Sequence of Cell-permeable Fluorogenic Caspase Substrates Used in This Study

SubstrateTarget caspaseActivitySequence
PhiPhiLuxCaspase 3/7DEVDaseKDPC5GDEVDGIDGC5PKGY
CaspaLux-3PEProcaspase 3 processingIETDaseKDPC5GIETDSGVGC5PKGY
CaspaLux-6Caspase 6VEIDaseKDPC5GLVEIDNGGC5PKGY
CaspaLux-9Caspase 9LEHDaseKDPC5GLEHDGINGC5PKGY
CaspaLux-1Caspase 1WEHDaseKDPC5GWEHDGINGC5PKGY
  • The amino acid residues constituting the major protease recognition motif (P4 to P1) are shown in boldface. Caspase cleavage occurs at the COOH end of the P1 amino acid. The IETD recognition sequence is preferred by caspase 8 also. The fluorophores are covalently conjugated through the ε-amino groups of lysine side chains. C5 is ε-aminocaproic acid.