Table 2

Development of Disease Correlates with a Significant Increase in the Total Number of Leukocytes and CD4+ Cells Recovered from the LP

Phenotype of CD4+ T cells used for reconstitutionmAb treatmentTotal no. of LP leukocytesTotal no. of CD4+ cells
×103 ± SEM×103 ± SEM
4 × 105 WT CD45RBhigh136.0 ± 34.992.7 ± 10.2
2 × 105 IL-10−/− CD45RBlow64.4 ± 29.743.9 ± 22.8
4 × 105 WT CD45RBhigh
+ 2 × 105 WT CD45RBlowanti–IL-10R176.3 ± 104.5133.3 ± 88.6
4 × 105 WT CD45RBhighIsotype
+ 2 × 105 CD45RBlowcontrol7.9 ± 0.95.4 ± 0.5
  • RAG-2−/− or C.B-17 SCID recipients were reconstituted with cell subsets as indicated. 8–12 wk after cell reconstitution, cells from LP were isolated. Data represent the number ± SEM of two to five animals per group from two independent experiments.