Table 1

The Function of Regulatory T Cells That Control Inflammatory Responses in the Colon Is Dependent on IL-10

Phenotype of CD4+ T cells injectedmAb treatmentNo or minimal colitis (0–1)Mild colitis (2)Severe colitis (3–5)
RAG-2−/− recipients
4 × 105 CD45RBhigh8/41 (19.5%)7/4126/41 (63.4%)
4 × 105 CD45RBhigh
+ 2 × 105 WT CD45RBlow26/31 (83.8%)3/312/31 (6.4%)
4 × 105 CD45RBhigh
+ 2 × 105 IL-10−/− CD45RBlow6/28 (21.4%)4/2818/28 (64.2%)
2 × 105 IL-10−/− CD45RBlow5/22 (22.7%)4/2213/22 (59.0%)
C.B-17 SCID recipients
4 × 105 CD45RBhigh0/93/96/9
4 × 105 CD45RBhigh
+ 2 × 105 CD45RBlowIsotype control6/71/70/7
4 × 105 CD45RBhigh
+ 2 × 105 CD45RBlowAnti–IL-105/50/50/5
4 × 105 CD45RBhigh
+ 2 × 105 CD45RBlowAnti–IL-10R0/80/88/8
4 × 105 CD45RBhighAnti–IL-10 +
+ 2 × 105 CD45RBlowAnti–IL-10R0/52/53/5
  • RAG-2−/− or C.B-17 SCID mice were reconstituted with sorted CD4+ T cell subsets and treated for 8 wk with antibodies as indicated (2 mg the day after T cell reconstitution and 1 mg/wk thereafter for anti–IL-10 mAb and isotype control; 1 mg the day after T cell reconstitution and 0.5 mg/wk thereafter for anti–IL-10R mAb). 8–12 wk after reconstitution, mice were killed and colonic pathology was graded on a scale of 0–5 as described in Materials and Methods.