Table 1

Unfamiliar Class II–bound Peptides Are Involved in CD4+ T Cell Division in Irradiated Hosts

Source of donor T cellsRecipientRecipient irradiatedNo. of independent experimentsPercentage of transferred CD4+ T cells that have undergone a given number of divisions
0 divisions1 division2 divisions
average ± SEM
B6.PLB6no597.0 ± 0.52.8 ± 0.40.3 ± 0.5
AbKOno496.8 ± 0.53.3 ± 0.30.0 ± 0.0
B6yes557.7 ± 2.938.9 ± 2.44.5 ± 0.9
AbKOyes479.2 ± 2.319.3 ± 2.21.3 ± 0.4
AbEpTKOAbEpTKOyes491.8 ± 4.67.5 ± 4.01.1 ± 0.8
TKOyes485.7 ± 9.813.3 ± 9.71.7 ± 0.9
AbEpTKOAbEpTKOyes196.6 ± 2.73.4 ± 2.60.8 ± 0.7
TKOyes190.2 ± 2.27.9 ± 2.13.8 ± 3.6
DO TCR + RAG2KOBALB/cyes389.6 ± 2.16.5 ± 1.02.6 ± 0.3
  • Donor T cells were purified and labeled with CFSE before transfer to untreated or irradiated recipients. 7 d after transfer, recipients were analyzed and the percentage of transferred CD4+ T cells that had not divided, divided once, or divided twice was determined using histogram markers as indicated in Fig. 3. In the experiment in which AbEpTKO donor mice were injected with SEB and LPS before transfer, the data shown indicate the percentage of Vβ8+CD4+ (Vβ8+ T cells are activated by SEB) cells that went through a given number of divisions.