Table 4

Reduced Number of B16-F10 Lung Metastases after Combination Treatment with Anti–CTLA-4 and F10/GM Vaccine

Treatment of lung metastasesLung metastasis count
Control hamster IgG>200, >200, >200, 25, 16
Anti–CTL-4>200, >200, >200, >200, >200
Hamster IgG plus F10/GM vaccine>200, >200, 35, 49, 4
Anti–CTLA-4 plus F10/GM vaccine87, 28, 6, 0, 0
  • B16-F10 (105, i.v.)-induced lung metastases were treated with hamster IgG, 9H10, and F10/GM vaccine in combination with either antibody on days 1, 4, and 7 after challenge. Surface lung metastases were counted under a dissecting microscope 25 d after inoculation. Counts are shown for each individual mouse; all counts over 200 were scored as >200.