Table I

Treatment with GK1.5 Anti-CD4 Antibody Transiently Depletes CD4+ T Cells in BALB/c Mice Infected for 3 wk with L. major

Days after GK1.5Percentage of CD4Percentage of CD8
 030.3 ± 1.912.6 ± 0.9
 35.4* (4.1)27.4* (22.5)
 7 5.9 ± 1.921.2 ± 0.7
2114.6 ± 1.120.4 ± 1.2
6318.621.4
  • Groups of five BALB/c mice were infected with L. major for 3 wk and injected with 1.0 mg of GK1.5 mAb. Spleen cells were harvested at the indicated number of days after injection with GK1.5 and the percentage of total cells expressing surface CD4 or CD8 was determined by two-color FACS® analysis. Shown are mean percentage of cells positive ± SEM (n = 4).  

  • *  Cells were pooled in one group and the parentheses indicate the results from a separate experiment where data represent pooled lymph node cells from four mice.