Table 1

VH Gene Sequences of IgD+CD27+ and IgD+CD27 B Cells

CD27 +CD27
CellVH genePot. funct.MutationsCellVH genePot. funct.Mutations
Donor 1no.%no.%
 D1/7DP46 (3)+ 3 1.5D1/2(Z80675) (3)+10.5
 D1/8V3-23+ 6 3.0D1/3V3-30+00
 D1/9V3-48+ 2 1.0D1/4V3-9+00
 D1/103d279d (4)+ 6 3.0D1/5V4-34+00
 D1/11V3-23+10 5.0D1/7V3-5300
DP70 (4)+ 5 2.63d279d (4)+00
 D1/12V3-7+ 4 2.0D1/8DP48 (3)+00
 D1/14V3-23+ 00D1/10V3-30+00
 D1/15DP50 (3)+ 6 3.0D1/11V3-30+00
 D1/16V3-9+ 0 0D1/13V3-1500
 D1/17DP46 (3)+12 6.0D1/14V3-11+00
 D1/19V3-21+ 3 1.5D1/16V3-11+00
 D1/20V3-5313 5.9D1/19DP64 (4)+00
 D1/22V3-53+12 6.7D1/20V3-23+00
 D1/23DP7 (1)+ 5 2.2D1/21LSG12.1 (3)+00
 D1/25V3-11 4 2.3D1/22DP46 (3)+00
 D1/26V3-48+2412.4D1/23V3-23+00
 D1/27V3-23+ 5 2.5D1/26DP10 (1)00
 D1/28DP29 (3)+13 6.5V3-23+00
 D1/29V3-53 6 3.4D1/27V3-15+00
4.30 (4)+12 6.1D1/29V3-30+00
 D1/30cos8 (3)+ 9 4.5
 D1/31DP71 (4)+ 5 2.6
 D1/32V3-23+11 5.6
Means 3.70
Donor 2
 D2/1DP50 (3)+13 6.5D2/1DP78 (4)+00
V4-4b11 5.6D2/2V3-7+00
 D2/2DP29 (3)+ 2 1.0D2/8DP88 (1)+00
 D2/3DP58 (3)+ 9 4.5D2/13V1-18+00
 D2/5V3-9+ 9 4.5D2/16V3-21+00
 D2/6V4-31 9 3.3D2/18V3-15+00
 D2/7V3-30+11 5.5D2/23V4-34+00
 D2/8V3-53+ 9 4.7D2/25V3-30+00
 D2/9DP67 (4)+ 6 3.1D2/26V4-31+00
V3-2320 9.0D2/28V3-9P+00
 D2/11DP67 (4)+3013.5D2/30V3-30+00
DP46 (3)2413.9
 D2/15DP58 (3)+ 9 4.5
 D2/17V3-9+ 1 0.5
 D2/18V1-18+ 9 4.5
DP46 (3)13 6.6Means0
 D2/19V3-15+ 10.5
 D2/20V3-15+115.6
VH4.16+ 0 0
 D2/23V4-34+10 5.2
 D2/25V3-30+ 3 1.5
 D2/26V3-23+1810.0
 D2/27V4-31+ 00
Means 5.0
Donor 3
 D3/1DP79 (4)+ 4 2.0
 D3/3V4-34+ 9 4.5
 D3/4V3-23+2612.9
 D3/5V3-23+2311.0
 D3/7V3-23+2412.7
DP79 (4)+14 7.1
 D3/8DP50 (3)15 7.9
3d279d (4)+11 5.5
 D3/9DP50 (3)14 7.1
 D3/10V4-4+ 1 0.5
 D3/12Yac-5 (3)+ 00
 D3/15V3-23+ 8 4.9
 D3/17V1-18+12 6.2
 D3/18V3-9 7 4.3
 D3/24DP75 (1)+ 7 4.0
 D3/27V3-22P13 8.0
DP75 (1)+ 5 3.0
 D3/28V3-13+15 8.9
 D3/32GLSJ2a (3)+ 6 3.6
 D3/33V3-7+ 8 4.8
Means 5.9
  • On average, 195 bp of the VH gene segment were sequenced. If possible, the VH gene nomenclature by Matsuda et al. was used (reference 51). For other genes, the VH family is indicated in parentheses after the gene name. Several sequences from CD27+ B cells harbored deletions and/or insertions in addition to point mutations: D1/20, 1-bp deletion in FRI and 1-bp deletion in CDRII; D1/25, 28-bp deletion in CDRII; D1/29 (V3-53), 11-bp deletion in CDRII; D1/32, 3-bp deletion in CDRII; D2/6 bp, two overlapping duplications (28 and 21 bp) in FRIII; D2/9 (V3-23), 1-bp deletion in CDRII; D2/11 (DP46), 17-bp duplication in FRI and 47-bp deletion in CDRII; D2/11 (DP67), 6-bp duplication in CDRII; D3/7 (V3-23), 3-bp deletion in CDRII; D3/8 (DP50), 6-bp deletion in CDRII; D3/27 (V3-22P), 34-bp duplication in JH. The sequences reported in this paper are available from EMBL/ GenBank/DDBJ under accession no. AJ231545AJ231685. Pot. funct., Potentially functional.