Table 1

Receptor Profile of Various APCs

Immature DCs Day 7Mature DCs Day 11Monocytes Day 0Macrophages Day 3Macrophages Day 9
n = 7n = 6n = 4n = 5n = 5
CD8*0–2 (3 ± 1)§0–3 (4 ± 1)0–1 (4 ± 1)0–5 (3 ± 1)1–2 (3 ± 1)
CD14*1–10 (4 ± 1)0–6 (4 ± 1)83–97 (149 ± 90)67–98 (61 ± 20)49–90 (68 ± 48)
CD83‡8–43 (8 ± 4)73–99 (114 ± 15)0–1 (3 ± 1)4–19 (4 ± 2)2–14 (4 ± 2)
MHC I‡99–100 (311 ± 49)96–100 (280 ± 69)99–100 (99 ± 38)97–99 (164 ± 24)95–100 (116 ± 18)
HLA-DR*88–100 (84 ± 35)98–99 (188 ± 40)94–99 (63 ± 9)97–99 (173 ± 34)5–89 (25 ± 27)
CD8‡3–9 (4 ± 1)2–8 (3 ± 1)0–2 (3 ± 1)1–4 (3 ± 0)1–5 (3 ± 1)
IgG2b‡3–7 (3 ± 1)2–7 (3 ± 1)0–2 (3 ± 0)1–4 (3 ± 1)1–5 (3 ± 1)
CD36‡75–99 (75 ± 31)20–50 (9 ± 2)89–99 (62 ± 18)64–98 (69 ± 45)9–55 (15 ± 16)
αv (CD51)‡45–76 (14 ± 3)11–40 (9 ± 3)2–12 (4 ± 1)2–35 (6 ± 2)18–75 (10 ± 5)
β1 (CD29)‡99–100 (287 ± 57)84–100 (112 ± 28)95–100 (61 ± 33)83–100 (72 ± 23)68–99 (104 ± 29)
β3 (CD61)‡14–34 (7 ± 2)10–24 (6 ± 1)76–91 (40 ± 16)‖15–85 (14 ± 9)‖19–92 (16 ± 9)
β578–95 (30 ± 8)32–57 (11 ± 2)6–8 (5 ± 1)8–15 (7 ± 2)5–8 (3 ± 1)
αvβ3 (VnR)‡22–28 (7 ± 1)14–30 (7 ± 2)4–8 (4 ± 1)7–27 (5 ± 2)19–68 (10 ± 4)
αvβ581–90 (25 ± 5)12–53 (9 ± 3)1–14 (4 ± 1)0–11 (3 ± 1)6–15 (4 ± 1)
CD71‡75–99 (50 ± 29)75–94 (61 ± 27)3–7 (4 ± 0)14–30 (7 ± 2)58–81 (30 ± 7)
Mannose receptor‡99–100 (272 ± 68)38–80 (26 ± 11)2–7 (4 ± 0)24–63 (13 ± 8)48–95 (34 ± 20)
  • * PE-conjugated.  

  • Unconjugated antibody.  

  • § Results are expressed as range of values, calculated as percentage of positive cells as compared with an isotype-matched control antibody. Values in parentheses indicate the average of geometric mean fluorescence intensities of the various samples ± SD.  

  • The β3 expression in the monocytes and day 3 macrophages does not match the αvβ3 expression due to contaminating platelets that stuck to the cells during isolation, as determined by staining with anti-CD41 (data not shown).